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KMID : 0358419930360113774
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Korean Journal of Obstetrics and Gynecology
1993 Volume.36 No. 11 p.3774 ~ p.3783
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The Effect of Peritoneal Macrophage on Progesterone and Estrogen Production of Follicular Granulosa Cells in Human
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조동제
장경환/조환승/박원일/이병석/박기현/송찬호
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Abstract
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To evaluate the action of macrophage on hormone production in human granulosa cell, we cultures cell with and without macrophage. The granulosa cells were obtained during oocyte retrieval in cases of IVF-EF. The peritoneal macrophages were
obtained
in
cul-de-sac of the same patients. The granulosa cell were cultured alone(control group), with hCG, and with macrophage. The concentration of hormones were measured in the media after 2, 4 and 6 days.
The production of progesterone was sustained after 6 days in hCG group. The progesterone production of granulosa cell co-cultured with macrophage was higher than control group up to 4 days, but was lower than hCG group. The estradiol production
decreased abruptly 2 days after incubation in every group. The concentraction of estradiol was slightly lower in macrophage co-culture group after 2 days, and was not different from other group after 4 and 6 days. The prostaglandin E2 production
decreased 2 days after incubation in every group. The concentration of PGE was significantly higher in macrophage co-culture group after 2 or 4 days, but this effect did not continue after 6 days.
From this dais, we can conclude that hCG has tropic effect on progesterone production of granulosa cell in vitro. Although macrophage showed similar tropic effect, the potency and duration were weaker and shorter than hCG. We think this hormone
producing effect of macrophage was probably due to the activation of some growth factors.
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KEYWORD
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